Based on the ship automatic identification system (AIS) data, the spatial-temporal feature of fishing vessels and commercial ships activities were identified，and the potential pressure caused by marine ships on ecological red line sea area and marine functional zone in Laizhou Bay were assessed by using GIS spatial analysis and geospatial simulation methods. Results show that maritime vessel activities in Laizhou Bay are concentrated in the southwest sea area and marine functional areas, such as port shipping area, agricultural fishery area and so on; the daily and seasonal variations of fishing vessels are more obvious; the potential pressure of the marine vessel activities in the southwestern sea area of the Laizhou Bay and the northwest of Longkou port area is greater. It is recommended to strengthen the supervision of fishing activities and fishing operations in Laizhou Bay in the 9 —11 months of the year. Ecological environment quality monitoring of marine ecological red line areas such as Yellow River Estuary and Laizhou shoal should be carried out, and prevent the harmful effect resulted from commercial vessel activities on the marine environment of culture zone and oceanic pasture surrounding the port and shipping zone.