In order to manage and coordinate the supply chain more effectively, the different effects of frequent demand disturbances on various coordination modes and stakeholders in the port logistics service supply chain were studied, a port logistics service supply chain model composed of ports and maritime carriers considering demand disturbances was constructed by using the two-stage game method, and the influence of demand disturbance on the decision-making of shipping agents under different coordination modes was studied. The results show that the distribution proportion of demand disturbance only affects the carrier’s service quality and price decision under the decentralized decision-making mode. When the service quality cost coefficient is large, the centralized decision-making model has higher robustness to the carrier price fluctuation, and when the service quality cost coefficient is small, the carrier alliance decisionmaking model can better reduce the impact of demand disturbance on the carrier price. Finally, through numerical analysis, this paper discussed the influence of demand disturbance and demand disturbance distribution proportion on the decision-making of port logistics service supply chain members under different modes.